Rapid detection of Salmonella without enrichment
Emily J. Harrington, Utah State University, United States
Utah State University . Awarded
Salmonella is one of the leading causes of foodborne illness today and is known for its presence in poultry products, including chicken and eggs. Because of this, rapid food pathogen detection systems are under immense research. The aim of this study was to eliminate the need for time-consuming enrichment steps. This was achieved by a rapid detection system that used anti-Salmonella-coated beads to capture and concentrate Salmonella for detection via PCR or an enzyme-linked immunosorbent assay (Rapid Immuno-Capture or RIC).
The RIC assay detected Salmonella spp. at concentrations in buffer as low as 4 × 101 CFU/ml and in chicken rinse and shell eggs as low as 4 × 103 CFU/ml. The antibody capture with PCR detected Salmonella at concentrations in buffer as low as 4 × 102 CFU/ml, in chicken rinse as low as 4 × 105 CFU/ml, and in shell eggs at 4 × 106 CFU/ml.
Harrington, E.J. Rapid detection of Salmonella without enrichment. Master's thesis, Utah State University.
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